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Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2011/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Targeted Suppression of Connexin 43 in Ovine Preimplantation Embryos by RNA Interference Using Long Double-stranded RNA
Connexin 43 RNA Interference Ovine Embryo
2010/5/4
RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of...
Ever since RNA interference was discovered, in 1998, scientists have been pursuing the tantalizing ability to shut off any gene in the body — in particular, malfunctioning genes that cause diseases su...
RNA干涉及其应用前景RNA Interference and Its Promising Future
RNA干涉 小干涉RNA 转录后基因沉默 Dicer酶 RdRP活性 功能基因组
2007/12/30
摘要RNA干涉是指由特定双链RNA(dsRNA)引起的转录后基因沉默现象。研究表明，Dicer断裂dsRNA产生的小干涉RNA可以抑制哺乳动物体细胞和胚胎中的基因的表达。 RdRP在扩增RNAi中起着关键性的作用,RdRP活性复制较长的触发性dsRNA或以一种非引物的方式复制短的siRNA，即以siRNA为引物的RdRP反应使靶mRNA转变为dsRNA，同时复制触发性dsRNA。 所有的产物又可作...
摘要RNAi是多种生物体内由dsRNA介导的同源mRNA降解现象。这是一个高度特异化的过程，涉及多种蛋白质的共同参与。在这一过程中，siRNA的结构影响其两条链装配到RISC中去的能力。除了与RISC结合外，siRNA还引导了RITS复合物结合到同源染色质，介导异染色质化过程。干扰效应的扩散，即系统性沉默可能依赖于跨膜蛋白的转运，并且很可能是在多因素调控下完成的。Abstract: RNA int...