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Biological arms races are commonplace in nature. Cheetahs, for example, have evolved a sleek body form that lends itself to rapid running, enabling them to feast upon similarly speedy gazelles, the fa...
On the evening of Dec. 12, 2021, Cornell astronomers Gordon Stacey and Thomas Nikola were dangerously short on time. They’d been awake for nearly 24 hours, with another all-nighter ahead of them.
When you’re frying something in a skillet and some droplets of water fall into the pan, you may have noticed those droplets skittering around on top of the film of hot oil. Now, that seemingly trivial...
Ateam of physicists from the Harvard-MIT Center for Ultracold Atoms and other universities has developed a special type of quantum computer known as a programmable quantum simulator capable of operati...
Serum caffeine and paraxanthine concentrations and menstrual cycle function: correlations with beverage intakes and associations with race, reproductive hormones, and anovulation in the BioCycle Study
anovulation biological markers caffeine menstrual cycle 1,7-dimethylxanthine
2016/5/25
Background: Clinicians often recommend limiting caffeine intake while attempting to conceive; however, few studies have evaluated the associations between caffeine exposure and menstrual cycle functio...
Graphene joins the race to redefine the ampere
Graphene joins the race redefine the ampere
2013/5/13
The world’s first graphene single-electron pump (SEP), described in a paper in Nature Nanotechnology, provides the speed of electron flow needed to create a new standard for electrical current based o...
It is being dubbed as one of the most effusive presidential primaries in a long time and one that will surely go down in political chronicles. U.S. Democratic presidential nominees Hillary Rodham Clin...
简并PCR结合RACE技术克隆申克孢子丝菌未知过氧化氢酶基因
孢子丝菌 过氧化氢酶 简并PCR 快速cDNA末端扩增
2011/5/24
目的 克隆孢子丝菌未知过氧化氢酶基因,命名为Sscat基因。方法 根据生物信息库中7种已知真菌过氧化氢酶氨基酸序列的高度保守区域设计简并引物,PCR扩增获得部分Sscat基因cDNA片段,随后应用RACE技术分别扩增其3’端和5’端未知序列。结果 Sscat基因cDNA序列全长1746 bp,其中包括5’端121 bp的非编码区、1500 bp的编码区和109 bp的3’端非编码区。该基因编码49...
Busy brain bees race to finals
2010 Brain Bee Champion the national finals neuroscience questions
2010/1/28
Ten of the brainiest high school students from across Australia and New Zealand will fight it out for the title of 2010 Brain Bee Champion at the national finals in Sydney this weekend.
The event ma...
Photo finish in race for strontium condensate（图）
Photo finish strontium condensate strontium atoms
2009/11/18
An Austrian group has beaten its US counterpart by a matter of days in a race to create a Bose-Einstein condensate (BEC) of strontium atoms.Researchers at the Institute of Quantum Optics and Informati...
Medicine, Race and the General Good：The Career of Thomas N G Te Water (1857–1926), South African Doctor and Medical Politician
South African Doctor Medical Politician Thomas N G Te Water General Good
2008/11/27
In a recent lecture on the Oxford Dictionary of Biography, Keith Thomas argued that an anti-heroic and democratizing age had subverted the “Great Man” tradition of biography, but that a modern biograp...
摘要为克隆马铃薯晚疫病抗性相关基因，深入研究马铃薯晚疫病抗性机制，应用SMART LD-PCR技术,以晚疫病菌混合小种诱导48 h的水平抗性马铃薯（Solanum tuberosum L.）(R-gene-free)叶片为材料，构建了一个富集晚疫病抗性相关基因的cDNA文库。为提高克隆全长cDNA的效率，将cDNA文库与RACE技术结合，依据本实验室得到的病原诱导表达片段测序结果，在其内部设计两个...
一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3’RACE
RACE 触减PCR MnSOD cDNA
2008/1/8
摘要
本研究尝试将触减 PCR与3’ cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术进行结合，仅用一条特异性引物和一条通用引物，成功地实现了从3’末端cDNA库对鸡含锰超氧化物歧化酶（manganese-containing superoxide dismutase，MnSOD）全长cDNA的一步3’RACE快速构建。与常规使用的末端PCR...
大多数有重要功能的蛋白质都含相应的由保守氨基酸顺序组成的功能结构域。本文首先根据蛋白质功能结构域保守氨基酸序列设计简并引物，用PCR方法扩增出基因EST序列，再利用改进的快速扩增cDNA末端（RACE）方法从cDNA文库中扩增出基因非同源部位，然后以非同源序列为探针，筛选cDNA文库。利用此方法成功地从人骨髓cDNA文库中克隆到几个编码锌指蛋白并代表原有EST的新的全长cDNA。这一策略也应适用于...