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为研究控制禾谷镰孢菌生长及抑制其脱氧雪腐镰刀菌烯醇（Deoxynivalenol，DON）产生的安全、有效生物防治技术，利用植物内生菌分离技术从小麦发病麦穗中分离得到的256株内生菌，通过平板对峙、共培试验，筛选到1株可显著抑制小麦赤霉病菌生长及降低DON含量的拮抗内生细菌YB-144。通过其形态特征、生化特性和16S rDNA序列比对，结果表明，菌株YB-144为贝莱斯芽孢杆菌，菌株YB-144...
禾谷镰刀菌Fgap1转录因子调控Tri基因表达及DON毒素合成
Fgap1转录因子 禾谷镰刀菌 DON毒素 调控机制
2019/4/28
DNA序列同源比对分析预测出镰刀菌氧胁迫调控因子Fgap1在DON合成基因簇的Tri5和Tri10基因上有结合位点。为阐明Fgap1转录因子在禾谷镰刀菌的Tri基因表达和DON产生中的作用,本研究以野生型PH-1和突变株⊿Fgap1为试验材料,通过菌落形态观察、菌丝生长速率、菌丝结构观察分析氧压敏感性,通过实时荧光定量PCR技术分析Tri基因在不用氧化压力下的表达量,并采用HPLC法测定DON毒素...
为明确不同杀菌剂防治小麦赤霉病和减少小麦籽粒中DON(脱氧雪腐镰刀菌烯醇)毒素积累的效果, 于大田人工接菌条件下进行了不同杀菌剂对小麦赤霉病的防治试验, 并于收获期测定了不同药剂处理的小麦籽粒中DON毒素含量。结果表明:两种处理方式下, 50%多菌灵WP, 30%多·酮SC, 25%戊唑醇WP, 25%氰烯菌酯SC和70%甲基硫菌灵WP处理中, 除先喷药后接菌条件下25%戊唑醇WP在病粒率和籽粒中...
戊唑醇不同防治时间对小麦赤霉病发生和籽粒中DON毒素积累的影响
小麦 小麦赤霉病 防治时期 脱氧雪腐镰刀菌烯醇
2017/5/16
为明确戊唑醇防治小麦赤霉病以及减少籽粒中DON (脱氧雪腐镰刀菌烯醇) 毒素积累的关键时期, 本研究于2015年和2016年在河南省焦作市温县进行了小麦花期前后不同时间喷施25%戊唑醇可湿性粉剂防治小麦赤霉病和减少籽粒中DON毒素积累的效果评价。试验结果表明:在2015年‘豫保1号’和2016年‘周麦18’上, 齐穗期 (Ⅰ-3, DAA=-1, Zadoks=59) 、始花期 (Ⅱ-1, DAA...
Mid-rotation response to fertilizer by Pinus radiata D. Don at three contrasting sites
forest fertilization sustained response volume increment stand growth foliar nitrogen
2016/5/11
Mid-rotation responses to fertilization of Pinus radiata D. Don plantations after the first or second thinning have been observed in research experiments in many regions where the species is grown. Th...
Soil-forming effect of Grand fir (Abies grandis [Dougl. ex D. Don] Lindl.)
forest ecosystems Grand fir introduction
2015/3/16
The aim of the study is to evaluate the role of the Grand fir (Abies grandis [Dougl. ex D. Don] Lindl.) as a soil-improving species in the area of the University Training Forest in Kostelec nad 萫rn齧i ...
Variation in the production of trichothecene mycotoxin deoxynivalenol (DON) in spring barley varieties after treatment with the fungicides azoxystrobin and tebuconazole
deoxynivalenol Fusarium culmorum azoxystrobin tebuconazole
2015/2/2
Eight varieties of spring barley (Hordeum vulgaris Lin.) were artificially inoculated with a Fusarium culmorum (W.G. Smith) Saccardo – isolate and naturally infected in the middle of the flowering per...
DON毒素在小麦赤霉病抗感品种麦穗组织中的积累分析
脱氧雪腐镰刀菌烯醇 小麦品种 赤霉病
2015/1/29
本研究采用单小花滴注和喷雾接种方法对3个抗病品种(‘望水白’、‘苏麦3号’、‘扬麦16’)和3个感病品种(‘周麦18’、‘矮抗58’、‘豫保1号’)的赤霉病抗性进行分析, 并用ELISA测定了籽粒、颖壳和穗轴中的DON毒素水平。结果表明:两种方法接种后第20天, 除‘望水白’外, 抗病品种的病级日扩展速率和病小穗率都显著低于感病品种(P＜0.05); 单小花滴注接种条件下, 除‘周麦18’的籽粒和...
Unequal Paternal Contribution in an Sugi (Cryptomeria japonica D. Don) Population Made by Mixed Pollen Using Microsatellite Markers
controlled pollination Cryptomeria japonica D.Don fertilization microsatellite markers
2009/8/28
We investigated paternal contribution in artificial crossed-populations made by mixed pollen from plus tree clones of Cryptomeria japonica D. Don in Niigata Prefecture. Using six polymorphic microsate...
Comparison of Changes in Organic Matter Dynamics due to Stand Age between Artificial Japanese Cedar (Cryptomeria japonica D. Don) Forests and Japanese Cypress (Chamaecyparis obtusa Sieb. et Zucc.) Forests
artificial Japanese cedar forest artificial Japanese cypress forest carbon mineralization rates of the litter organic matter dynamics stand age
2009/8/28
The purpose of this study was to clarify the effects of tree species on organic matter dynamics according to stand age in Kanto region of Japan. We investigated forest floor cover percentages, amounts...
Discrimination of Sugi (Cryptomeria japonica D. Don) Plus-tree Clones Using SSR Marker Analysis
clone discrimination discrimination power SSR marker sugi plus tree
2009/8/28
Eight hundred and thirty-five sugi (Cryptomerica japonica D. Don) plus-tree clones from the Kanto Breeding Region were genotyped using three highly polymorphic SSR markers. With a mean observed hetero...
Characteristics and Effectiveness of Glasshouse Miniature Seed Orchards in Cryptomeria japonica D. Don
Cryptomeria japonica D.Don glasshouse miniature seed orchard pollen contamination
2009/8/28
We produced seeds of Cryptomeria japonica trees in a glasshouse miniature seed orchard to prevent pollen contamination. In the glasshouse, male flowers shed pollen from February 4 to March 27, 2002, w...
Mechanism of Female Sterility in Sugi (Cryptomeria japonica D. Don)
Cryptomeria female sterility male sterility megaspore mother cell
2009/8/28
Two C. japonica trees exhibiting male sterility were found in Niigata Prefecture, Japan. These trees exhibited not only male sterility but also female sterility, because their seeds did not germinate....
An Investigation on the Allocation of Selection Weight on Growth and Wood Basic Density to Maximize Carbon Storage in the Stem of Sugi (Cryptomeria japonica D.Don) Plus-tree Clones
carbon storage clone global warming prevention index selection sugi
2009/8/27
An optimum allocation of selection weight on the traits that were related to carbon storage in the stem of sugi was investigated to establish criteria for choosing plus tree clones suitable for carbon...
Cytological and Genetical Studies on Male Sterile Sugi (Cryptomeria japonica D.Don),Shindai 1 and Shindai 5
Cryptomeria inheritance male sterility pollen
2009/8/24
Male sterile sugi (Cryptomeria japonica D. Don) trees, “Shindai 1,” “Shindai 5,” were found in Niigata Prefecture, Japan. There were no significant differences in the appearances of male flowers and n...