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Premature capacitation of bovine spermatozoa is initiated by cryopreservation
Premature capacitation bovine spermatozoa cryopreservation
2009/2/17
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...
Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge
Milk egg yolk-Tris indo-1 acetoxymethyl ester cold
2009/2/13
Bovine spermatozoa are commercially cryopreserved by diluting the cells in media, known as extenders, followed by slow cooling and freezing. Previous work has shown that this process of cryopreser...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Actin and actin-binding proteins in bovine spermatozoa：potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
proteins bovine spermatozoa potential role signaling epididymal maturation
2008/12/26
The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells, including shape, movement, and interactions with extracellular matrices. The role of actin in mammalian male ger...
Acidification of intracellular pH in bovine spermatozoa suppresses motility and extends viable life
Acidification of intracellular pH bovine spermatozoa motility viable life
2008/12/25
Intracellular pH (pHi) was determined in ejaculated bovine spermatozoa using a ratiometric absorbance technique under various incubation conditions that drastically altered sperm motility. The pHi w...
GM1 Dynamics as a Marker for Membrane Changes Associated With the Process of Capacitation in Murine and Bovine Spermatozoa
Rafts ganglioside cholesterol cholera toxin annulus
2008/12/24
We previously showed that in live murine and bovine sperm heads, the ganglioside GM1 localizes to the sterol-rich plasma membrane overlying the acrosome (APM). Labeling GM1 using the pentameric c...
The Response of Bovine Spermatozoa to Bicarbonate and Its Use to Assess the Influence of Added Oviductal Epithelial Proteins on Cryopreservation
Isthmus ampulla motility sperm-oviduct interactions
2008/12/24
The oviduct is a crucial organ for fertilization and has been demonstrated to perform a variety of interactions with spermatozoa ranging from sperm storage, to stabilizing sperm membranes and red...
Examination of the Binding Ability of Bovine Spermatozoa to the Zona Pellucida as an Indicator of Fertility
Fertilization oocyte accessory cattle nonreturn heterospermic
2008/12/8
Despite the development of many new techniques, laboratory assays still do not predict male fertility accurately. To identify targets for laboratory assessment, we first need to determine which step...