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Polyamines in ejaculated ram spermatozoa and their relationship with sperm motility
Ram ejaculated spermatozoa polyamines sperm motility
2009/2/10
Intracellular polyamine levels in ejaculated spermatozoa and seminal fluid from rams were determined by fluorescent spectroscopy of their dansyl derivatives. Relationships between the sperm polyamin...
Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system
ram spermatozoa X and Y chromosome centrifugal countercurrent aqueous two-phase system
2008/12/25
The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic o...
Assessment of the acrosomal status of ram spermatozoa by RCA lectin-binding and partition in an aqueous two-phase system
acrosomal status ram spermatozoa RCA lectin-binding partition aqueous two-phase system
2008/12/25
The acrosome reaction is an important marker for sperm function. Because different laboratory techniques may be used to detect this exocytotic process, the objective of this study was to investiga...
Effect of Seminal Plasma Fractions From Entire and Vasectomized Rams on the Motility Characteristics, Membrane Status, and In Vitro Fertility of Ram Spermatozoa
Seminal Plasma Fractions Entire Vasectomized Rams Motility Characteristics Membrane Status
2008/12/24
Whole seminal plasma from ram semen collected before and after vasectomy was separated into 2 fractions, supernatant and pellet of vesicles, and their protein profiles characterized by one-dimens...
Comparative Study of Four Different Sperm Washing Methods Using Apoptotic Markers in Ram Spermatozoa
Swim-up caspase activity annexin V
2008/12/24
The accurate measurement of semen fertilizing potential is of great importance in determining the acceptability of processed semen for breeding purposes. A good sperm preparation technique result...
Immunocytochemical Localization and Biochemical Characterization of Two Seminal Plasma Proteins That Protect Ram Spermatozoa Against Cold Shock
Immunocytochemistry capacitation acrosome reaction
2008/12/23
We have already shown that seminal plasma proteins in the ram can repair cold-shock sperm membrane damage and that the addition of seminal plasma proteins before cold-shock treatment prevents sperm ...