搜索结果: 1-3 共查到“医学 tetraspanin”相关记录3条 . 查询时间(0.07 秒)
目的 扩增并表达日本血吸虫表膜蛋白(SjTsp2-A)基因 SjTsp2。 方法 根据与曼氏血吸虫表膜蛋白 SmT-sp2 基因同源的SjTsp2 序列(编号为AY810722)设计引物, 体外扩增目的蛋白基因,并将其亚克隆入原核表达载体pET28a, 转化至大肠埃希菌 (E. coli) BL21株, 异丙基-β-D-硫代半乳糖苷(IPTG)进行诱导表达, 用纯化的重组蛋白免疫小鼠, ELISA...
采用PCR法扩增日本血吸虫体表四跨膜家族蛋白2-A（SjTsp2-A）基因，构建重组质粒pcDNA3.1（+）/SjTsp2-A，将其转至大肠埃希菌DH5α制备DNA疫苗pcDNA3.1（+）/SjTsp2-A。24只BALB/c小鼠均分3组，每鼠于左股四头肌注射0.5 mg/ml盐酸布比卡因50 μl。次日，A组同法注射DNA疫苗pcDNA3.1（+）/SjTsp2-A，B组注射重组质粒pcDN...
Expression and Function of Transmembrane-4 Superfamily (Tetraspanin) Proteins in Osteoclasts: Reciprocal Roles of Tspan-5 and NET-6 during Osteoclastogenesis
cell fusion NET-6 osteoclast tetraspanin Tspan-5
2007/12/27
These data indicate that a diversity of tetraspanins is expressed in osteoclast precursors, and that cell fusion during osteoclastogenesis is regulated by cooperation of distinct tetraspanin family pr...