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Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Electrophoretic and Chromatographic Properties of Boar Sperm Plasma Membranes:Antigens and Polypeptides with Affinity for Isolated Zonae Pellucidae
sperm plasma membrane zonae pellucidae
2009/2/19
Two methods were used to assess the ability of plasma membrane (PM) polypeptides to bind to isolated zonae pellucidae (ZP). Crossed immunoelectrophoresis patterns of detergent solubilized PM were co...