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Sex steroid inhibition of LH induction of the LH receptor
estrogen testosterone luteinizing hormone receptor testis Leydig cell
2009/2/23
Testosterone and estrogen were tested as possible mediators of the prolactin-dependent LH induction of LH receptors in hypophysectomized adult male rats. Animals were injected s.c. with saline, pr...
Characteristics of prolactin-modulated LH induction of LH/hCG receptors. Transient inhibition of receptor induction following prolactin exposure
LH luteinizing hormone
2009/2/23
The temporal relationship between exposure to prolactin (PRL) and luteinizing hormone (LH) induction of LH receptors was investigated in hypophysectomized adult male rats. Testicular homogenate me...
Inhibition of ornithine decarboxylase activity by follicle stimulating hormone in primary culture of rat Sertoli cells
ornithine decarboxylase follicle stimulating
2009/2/23
The effect of follicle stimulating hormone on the activity of ornithine decarboxylase (ODC) was determined in primary culture of rat Sertoli cells. Three different FSH preparations (NIH oFSH-S-15, S...
Tonic inhibition of adenylate cyclase in cultured hamster Sertoli cells
cAMP hamster Sertoli cell adenylate cyclase culture pertussis toxin
2009/2/23
Sertoli cells cultured from immature hamsters respond to FSH with a dose-related increase in cAMP accumulation. Pertussis toxin acts synergistically with FSH to stimulate cAMP accumulation. This eff...
Cholinergic inhibition of cAMP accumulation in Sertoli cells cultured from immature hamsters
Sertoli cell cAMP muscarinic receptors FSH hamsters testis
2009/2/23
Acetylcholine inhibits FSH-induced cAMP accumulation in cultured Sertoli cells from immature hamsters. This action of acetylcholine is mimicked by muscarinic cholinergic agonists with a rank order o...
Kinetic characterization of the inhibition of purified cynomolgus monkey lactate dehydrogenase isozymes by gossypol
Kinetic characterization purified cynomolgus monkey dehydrogenase isozymes gossypol
2009/2/23
This report describes the results of the first step in a sequence of experiments designed to test the hypothesis that the sperm-specific isozyme of lactate dehydrogenase (LDH-C4), is a site of actio...
Racemic gossypol is an effective male antifertility agent in several mammalian species. However, (+)-gossypol is not an effective male antifertility agent in the rat or the hamster. Previous studies...
Specific inhibition of the testicular mitochondrial respiratory chain in vitro by gossypol
Specific inhibition testicular mitochondrial respiratory chain gossypol
2009/2/20
Optically inactive gossypol is an effective male antifertility agent in several mammalian species, while optically active (+)-gossypol has no antifertility effect in the rat and hamster. Recently, i...
Bovine sperm adenylate cyclase. Inhibition by adenosine and adenosine analogs
adenosine epididymal spermatozoa adenylate cyclase inhibition P-site
2009/2/20
Since both adenosine and cAMP affect sperm motility and metabolism, the authors have studied the mode of interaction of adenosine with the adenylate cyclase activity in either the presence or the ab...
Inhibition of 5 alpha-reductase activity and alteration of nuclear testosterone.
5a-reductase fibroblast androgen receptor enzyme inhibitor nuclear uptake
2009/2/20
17 beta-N, N-diethylcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one (4-MA) inhibits 5 alpha-reductase activity in cultured human genital skin fibroblasts. Enzyme kinetic studies analyzed by Eadie...
Fertility Inhibition In Vitro and Preliminary Antigen Identification
in vitro fertilization anti-sperm antibodies
2009/2/19
Rabbit anti-mouse sperm (MS) Fab fragments have been prepared, which are capable of inhibiting spermzona interaction (both binding and penetration) and sperm-egg fusion in vitro. In cumulus-intact...
Inhibition of Spermatogenesis and Steroidogenesis During Long-Term Treatment with hCG in the Rat
hCG spermatogenesis steroidogenesis
2009/2/19
The effects of chronic treatment with hCG (100 I.U.s.c. every second day) on testicular morphology, LH receptor levels and concentration of steroid intermediates of the 4 and 5 biosynthetic pathwa...
Estradiol-induced Inhibition of Testosterone Production by Testicular Interstitial Cells Incubated In Vitro
testosterone interstitial cells estradiol testis steroidogenesis
2009/2/18
Testosterone production by testicular interstitial cells incubated in vitro in the presence of 10-8 to 10-6 M 17-estradiol (E2), 17 -estradiol-3-benzoate (E2B), or benzoic acid (B) was examined. Hig...
The human acrosome reaction is highly sensitive to inhibition by cyclodiene insecticides
Sperm fertilization chiordane endosulfan
2009/2/17
The mammalian sperm acrosome reaction (AR) is essential to fertilization. It can be initiated in vitro by progesterone, a putative physiological initiator that helps to activate sperm GABA(A) recept...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...