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Comparison of a direct and indirect ELISA for quantitating antisperm antibody in semen
direct indirect ELISA quantitating antisperm antibody semen
2009/2/23
A direct and an indirect quantitative ELISA for antisperm antibody were compared using the spermatozoa and cell-free seminal fluid of 66 infertile males. The normal concentration of sperm binding immu...
Evidence that estrogen regulation of testosterone secretion in adult rams is mediated by both indirect（gonadotropin dependent）and direct（gonadotropin independent）means
estrogen regulation testosterone secretion adult rams
2009/2/23
Involvement of endogenous estrogen in the regulation of gonadotropin and testosterone secretion was investigated in adult rams. Groups of four rams were either passively immunized against estradio...
Temporal Dissociation of Feedback and Direct Testicular Effects of Estradiol Benzoate in Male Rats
estradiol negative feedback direct testicular effects testicular incubations
2009/2/19
Studies were conducted to characterize the responses of the hypothalamic-pituitary-testicular axis to systemic injection of estradiol benzoate (EB) in the rat. Feedback effects of estrogens on the...
Evidence for Direct Transfer of 1,2-3H Testosterone From Testicular Veins to the Rete Testis in Rats
spermatic vein rete testis fluid
2009/2/19
To investigate the origin and route of entry of testosterone into the rat rete testis, samples were drawn from the rete, deep testicular veins, testicular lymph and systemic arteries during testic...
Validation of a Direct Radioimmunoassay for Testosterone in Unextracted Serum from Five Species: Application to Study of the Hypothalamic-Pituitary-Gonadal Axis in Males
direct radioimmunoassay serum testosterone luteinizing hormone releasing hormone immunoneutralization
2009/2/19
A method for the radioimmunoassay of serum testosterone that does not require extraction or chromatography is described. Utilization of a highly specific antiserum, tritiumlabeled ligand, and doub...
A new method to generate canine seminal emission and its application to men：direct electrical stimulation of the vas deferens
generate canine seminal emission direct electrical stimulation vas deferens
2009/2/12
Seminal emission from the ejaculatory duct (SEED) by direct electrical stimulation of the vas deferens was investigated in the dog, and the technique was applied to men. The stimulus parameters used w...
In vivo model for chronic direct intratesticular drug administration
Male infertility testicular toxicity miniosmotic pumps
2009/2/12
The efficacy and toxicity of a new method for chronic direct intratesticular drug infusion were assessed in a rat model. To this end, luteinizing hormone (LH) or buffer was infused via miniosmotic...
The direct pituitary effect of testosterone to inhibit gonadotropin secretion in men is partially mediated by aromatization to estradiol
LH FSH bioactivity androgens estrogens DHT
2009/2/12
In men, administration of exogenous testosterone (T) exerts direct negative feedback effects at the pituitary as well as at the hypothalamic level. This study was undertaken to determine whether T i...
Direct and indirect effects of murine interleukin-2, gamma interferon, and tumor necrosis factor on testosterone synthesis in mouse Leydig cells
murine interleukin-2 gamma interferon tumor necrosis factor testosterone synthesis
2009/2/11
It was recently observed that treatment of patients with a high dosage of human interleukin (IL-2) resulted in suppression of plasma concentrations of testosterone. A murine model was developed to a...
Does ethane 1,2-dimethanesulphonate（EDS）have a direct cytotoxic effect on the seminiferous epithelium of the rat testis?
spermatogenesis cytotoxic testosterone testes rat
2009/2/6
The authors examined the possibility that ethane 1,2-dimethanesulphonate (EDS) has a cytotoxic effect on spermatogenesis that is not secondary to androgen withdrawal resulting from the well known cy...