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Little is known about the reorganization capacity of water molecules at the active sites of enzymes and how this couples to the catalytic reaction. Here, we study the dynamics of water molecules at th...
A nitrile-labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations. Ribonuclease...
The charge redistribution upon photoexcitation is investigated for a series of pyrene photoacids to better understand the driving force behind excited-state proton-transfer processes. The changes in e...
We report the first measurement of the vibrational Stark effect in a protein, providing quantitative information on the sensitivity of a vibrational transition to an applied electric field. This can b...
The electronic absorption line shape and Stark spectrum of the lowest energy Q(y)() transition of the special pair in bacterial reaction centers contain a wealth of information on mixing with charge t...
Stark Spectroscopy of Donor-Acceptor Polyenes:Correlation with Nonlinear Optical Measurements.
The Stark effect or electroabsorption spectrum of the Qy transition of the special pair, P, of Rb. sphaeroides reaction centers has been measured at 1.5 K in frozen glycerol/buffer glasses. Under thes...
The infrared spectra of CO bound to human myoglobin and myoglobin mutants at positions His-64, Val-68, Asp-60, and Lys-45 on the distal side have been measured between 100 and 300 K. Large differences...
The effects of an applied electric field on the Q y absorption and fluorescence spectra of three antenna complexes from photosynthetic bacteria have been measured at 77 K: the bacteriochlorophyll a pr...
The effect of an electric field has been measured on the absorption spectrum (Stark effect) of the heterodimer mutant (M)H202L of Rhodobacter sphaeroides reaction centers, where the primary electron d...
The nature of the initially excited state of the primary electron donor or special pair has been investigated by Stark effect spectroscopy for reaction centers from the photosynthetic bacteria Rhodops...
We have evaluated the application of spectroscopy using an insertion-type fiber-optic probe and a sensor at wavelengths from 400 to 1,100 nm to the measurement of milk fat content on dairy farms. The ...
Since the application of relatively high levels of heavy metals in the compost poses a potential hazard to plants and animals, the content of heavy metals in the compost with animal manure is importan...

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