搜索结果: 1-15 共查到“生物工程 METHOD”相关记录15条 . 查询时间(0.198 秒)
中国科学院动物研究所专利：Fusion protein for inducing pluripotent stem cells and application method thereof
中国科学院动物研究所 专利 哺乳动物 YAP蛋白 转录控制活性
2024/5/31
提供了由哺乳动物YAP蛋白或具有转录控制活性的片段的融合蛋白的与细胞全能性和转录激活结构域相关的基因编码的蛋白、编码核苷酸序列、表达载体及其组合物，以及使用该融合蛋白诱导多能干细胞的方法。
One of the most expensive steps in manufacturing protein drugs such as antibodies or insulin is the purification step: isolating the protein from the bioreactor used to produce it. This step can accou...
In recent years, scientists who study gene expression in cells have used a method that essentially pins a tail on RNA and tracks their whereabouts. However, certain types of RNA evade the process. Now...
Study shows limitations of method for determining protein structure（图）
Study limitation determine protein structure
2019/10/15
A new study by chemists at the University of Arkansas shows that X-ray crystallography, the standard method for determining the structure of proteins, may provide inaccurate information about a critic...
Computational Method Makes Gene Expression Analyses More Accurate
Computational Method Gene Expression Analyses More Accurate
2017/3/6
A new computational method can improve the accuracy of gene expression analyses, which are increasingly used to diagnose and monitor cancers and are a major tool for basic biological research.Research...
Lehigh researchers have successfully demonstrated the first precisely controlled, biological way to manufacture quantum dots. This one-step method starts with engineered bacterial cells in a simple, a...
Regulocator: A Novel Method for the Identification of Conserved Elements in Genomic DNA
ReguLocator DNA Conserved Elements
2015/5/26
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
Press Release: Computational Method Dramatically Speeds Up Estimates of Gene Expression, CMU, UMD Researchers Report
Press Release Computational Method Dramatically Speeds Estimates of Gene Expression CMU UMD Researchers Report
2014/4/21
PITTSBURGH—With gene expression analysis growing in importance for both basic researchers and medical practitioners, researchers at Carnegie Mellon University and the University of Maryland have devel...
Computational method dramatically speeds up estimates of gene expression
gene expression RNA sequencing data
2014/4/21
With gene expression analysis growing in importance for both basic researchers and medical practitioners, researchers at Carnegie Mellon University and the University of Maryland have developed a new ...
A Bayesian Method for Detecting and Characterizing Allelic Heterogeneity and Boosting Signals in Genome-Wide Association Studies
Bayesian Method Detecting Characterizing Allelic Heterogeneity Boosting Signals Genome-Wide Association Studies
2010/10/22
The standard paradigm for the analysis of genome-wide association studies involves carrying out association tests at both typed and imputed SNPs. These methods will not be optimal for detecting the si...
一种早期胚胎体外培养新方法的建立Establishment of a New Method for Early Embryo Culture in vitro
体外培养 套皿法 早期胚胎
2008/1/8
摘要
建立一种不依赖于液体石蜡的早期胚胎体外培养方法——套皿法，并比较了4种不同处理方法体外培养胚胎的效果。结果显示，采用套皿法进行胚胎培养，盖液体石蜡和不盖液体石蜡皿中的胚胎在各阶段的发育率差异不显著。与套皿法相比，用单皿微滴覆盖法(Brinster法)培养的胚胎在各阶段的发育率显著降低。不覆盖液体石蜡单皿中的胚胎则阻断于二细胞阶段。本实验设计的套皿法是一种有效的早期胚胎体外培养方法，为早期...
灰树花总DNA的制备及基因组文库的构建A Method for Preparation of Genomic DNA FromGrifola frondosa and Construction of a Genomic Library
灰树花 DNA提取 基因组文库
2008/1/8
摘要
灰树花是一种珍贵的药用真菌，因为多糖含量较高，较难获得高质量的总DNA，本文提出了一种制备高质量灰树花总DNA及构建灰树花基因组文库的方法。该方法制备的灰树花总DNA，经Sau3AI酶切后，用于构建基因组文库，可得到2×105个转化子/50mg，平均插入片段为14kb。本研究为下一步克隆灰树花中的基因以及进行其他分子生物学研究奠定了基础。Abstract： Grifola frondos...
采用一种不需要限制核酸酶和连接酶的新方法——“PCR一步法”将芳香烃化合物降解的关键基因pheB和绿色荧光蛋白编码基因gfp融合，构建得到融合蛋白基因fpg。该方法在一个PCR反应体系中通过三个引物、两个模板扩增得到一个含有中间柔性肽段-Gly4Ser-的融合基因fpg。本文研究结果表明，PCR一步法是一种快速方便的构建融合基因的方法。Abstract: TP-PCR，a method dev...
期刊信息
篇名
A fractal method to distinguish coding and noncoding sequences in a complete genome based on a number sequence representation
语种
英文
撰写或编译
撰写
作者
Li-Qian Zhou,Zu-Guo Yu,Ji-Qing Deng,Vo Anh,Shun-...
期刊信息
篇名
An ensemble method for gene discovery based on DNA microarray data
语种
英文
撰写或编译
作者
Li X,Rao S,Zhang T,Guo Z
第一作者单位
刊物名称
Science in China (C), English Edition
页面
2004, 47:396-405
出版日期
2004年
月
日
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