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Characterization of the Mitochondrial Proteome in Pyruvate Dehydrogenase Kinase 4 Wild-Type and Knockout Mice
Starvation Mitochondria Proteomics PDK4
2015/5/25
The goal of this study was to determine the effect of a PDK4 (pyruvate dehydrogenase kinase isoenzyme 4) knock-out on mitochondrial protein expression. A 2-D gel based mass spectrometry approach was u...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白（β-lactoglobulin,BLG）基因，扩增出的两个片段分别克隆到T载体上，利用BLG基因序列自身存在的NarI单酶切位点进行拼接，获得了全长为7.2kb的山羊BLG基因克隆，并构建了它的真核表达载体，经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠，经PCR和Southern印迹分析...
专著信息
书名
Systemic infusion of Flk1 mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice.
语种
英文
撰写或编译
撰写
作者
Fang B,Shi M,Zhao RCH
第一作者单位
出版社
Transplantation. 15;78(1):8...
May 21, 2007，Scientists often study mice as a model for human biology and disease, because their basic biological processes are assumed to be essentially the same as those of humans.But now, a team of...