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［目的］为鹦鹉热衣原体的快速、准确检测奠定基础。［方法］根据鹦鹉热衣原体主要外膜蛋白(MOMP)基因序列设计一对特异性引物，以SYBR GreenⅠ为荧光染料，建立了鹦鹉热衣原体Realtime quantitative PCR检测方法。根据检测结果，计算样品的批内和批间变异系数（CV）。［结果］以提取的鹦鹉热衣原体DNA为模板，用引物Cps1和Cps2进行PCR扩增，得到长100 bp...
獐茅AlHAK1 Real-time PCR定量方法的建立
獐茅 AlHAK real-time PCR mRNA
2009/6/11
本研究以β-actin为内参基因，根据本实验室克隆的AlHAK1及β-actin 碱基序列，分别设计了实时定量特异性引物，优化了反应的退火温度与引物浓度，并以优化的条件建立了相对定量标准曲线，同时对该方法的稳定性进行了评价。结果表明AlHAK1及β-actin基因的real-time PCR扩增效率分别为1.09和1.04，线性相关系数分别为0.996和0.997，批内及批间变异系数< 5 %。所...
Comparison of deep seepage estimations of a virtual with a real lysimeter by means of TDR-measurements
deep seepage TDR measurements lysimeter unsaturated hydraulic conductivity
2008/11/21
The virtual lysimeter concept was tested in comparison with a real lysimeter and found to be suitable for quantifying effective deep seepage dynamics in sandy soils. Discharge measurements and calcula...
Embedded real-time system for climate control in a complex greenhouse
greenhouse climate control embedded real-time systems statecharts integrated development Java
2008/11/19
This paper describes the development of an embedded real-time system devoted to microclimate control in a complex greenhouse. The control system is capable of managing multiple, independent poly-tunne...