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Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay
Acrldine orange chromatin structure SCSA TdTA
2009/2/13
Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the ...
Infertility is a growing problem among couples trying to conceive; in the past the female partner was singled out as the primary reason for being unable to bear a child. Research now reveals that ...
Reduction of the Incidence of Sperm DNA Fragmentation by Oral Antioxidant Treatment
Sperm DNA damage ejaculated spermatozoa TUNEL assay in vivo treatment vitamin C
2008/12/23
Sperm DNA fragmentation is known to compromise male fertility. Previous findings have suggested the implication of oxidative stress in the etiology of this pathological condition. The present study wa...
Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
Artificial insemination semen quality motility chlortetracycline viability SCSA
2008/12/22
We hypothesized that cryopreservation and incubation in conditions that mimic the female genital tract following insemination increases the susceptibility of ram sperm DNA to denaturation. Ram spe...
High Levels of Sperm DNA Denaturation as the Sole Semen Abnormality in a Patient After Chemotherapy for Testis Cancer
Sperm DNA Denaturation Sole Semen Abnormality Patient Chemotherapy Testis Cancer
2008/12/19
Systemic chemotherapy for the treatment of cancer is known to adversely affect male fertility potential. Sperm DNA damage is reported after cancer chemotherapy and is associated with semen 0abnormalit...
Male Genital Tract Antioxidant Enzymes: Their Source, Function in the Female，and Ability to Preserve Sperm DNA Integrity in the Golden Hamster
Superoxide dismutase glutathione peroxidase catalase sperm DNA damage oxidative stress Syrian hamster
2008/12/19
Recently, we reported that male accessory sex gland (ASG) secretions protect sperm genomic integrity by demonstrating that DNA damage was more extensive in sperm not exposed to the secretions. The p...
The Sperm Chromatin Dispersion Test: A Simple Method for the Determination of Sperm DNA Fragmentation
Structure human sperm decondensation
2008/12/18
Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation...
Effect of Leukocytospermia on Sperm DNA Integrity: A Negative Effect in Abnormal Semen Samples
Sperm quality DNA integrity leukocytes oxidative stress
2008/12/15
Controversy exists over levels of DNA integrity in the sperm of fertile and infertile men. In addition, the effect of leukocytospermia on sperm DNA in these 2 groups is unclear. We decided to address ...