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Vasopressin receptors in human seminal vesicles：identification, pharmacologic characterization, and comparison with the vasopressin receptors present in the human kidney
Vasopressin receptors human seminal vesicles identification pharmacologic characterization
2009/2/23
Because of the presence of a high density of vasopressin receptors in the epithelial cells of porcine seminal vesicles similar to the V2 vasopressin receptors of renal tubules, human seminal vesicle...
Purification and characterization of a sperm motility inhibitor in human seminal plasma
sperm motility inhibitor human seminal plasma
2009/2/23
A sperm motility inhibitor from human seminal plasma was purified and characterized. The purification procedure includes dialysis, ion exchange chromatography on SP-Sephadex C-25 and adsorption chro...
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
Characterization and localization of beta-adrenergic receptors in control and cryptorchidized rat testis by in vitro autoradiography
$-adrenergic receptors autoradiography cryptorchidectomy rat testis Sertoli cells
2009/2/23
beta-adrenergic receptors were localized and characterized in control and cryptorchidized rats to investigate further their role in testicular function. Slide-mounted cryostat sections were incubate...
Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails
Further characterization secreted epididymal glycoprotein mice sperm tails
2009/2/23
Sperm maturation antigen 4 (SMA-4) is a surface component of the mouse sperm tail. Previously, immunofluorescence studies indicated that SMA-4 may be secreted by principal cells of the distal caput ...
Characterization of beta-adrenergic receptors in dispersed rat testicular interstitial cells
rat testis Leydig cell $-adrenergic receptor
2009/2/23
Recent studies have shown that beta-adrenergic agents stimulate steroidogenesis and cyclic AMP formation in mouse Leydig cells in culture. To obtain information about the possible presence and the c...
Kinetic characterization of the inhibition of purified cynomolgus monkey lactate dehydrogenase isozymes by gossypol
Kinetic characterization purified cynomolgus monkey dehydrogenase isozymes gossypol
2009/2/23
This report describes the results of the first step in a sequence of experiments designed to test the hypothesis that the sperm-specific isozyme of lactate dehydrogenase (LDH-C4), is a site of actio...
Growth and characterization of polarized monolayers of epididymal epithelial cells and Sertoli cells in dual environment culture chambers
polarized monolayers epididymal epithelial cells Sertoli cells dual environment culture chambers
2009/2/23
Epididymal epithelial cells isolated from mature rats and Sertoli cells isolated from 10-day-old rats were cultured in serum-free defined media on extracellular matrix impregnated filters maintained...
Identification of arginine esterase as the major androgen-dependent protein secreted by dog prostate and preliminary molecular characterization in seminal plasma
arginine esterase dog prostate seminal
2009/2/20
This work was undertaken to determine the identity of the major androgen-dependent 15,000 molecular weight protein previously observed on SDS polyacrylamide gel electrophoresis of both dog prostate ...
Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Characterization of the Sperm Penetration Bioassay
hamster egg bioassay human sperm infertility zona-free
2009/2/19
Parameters influencing the zona-free hamster egg bioassay for human sperm fertility potential were evaluated. Egg recovery from hamsters superovulated at random with respect to their estrous cycle w...
Characterization of Cholinomimetic Agents that Inhibit In Vitro Fertilization in the Mouse
sperm binding cholinomimetics
2009/2/19
Spermatozoa from the cauda epididymidis of the mouse bind 3-quinuclidinyl benzilate (QNB), a highly specific antagonist of the muscarinic type of acetylcholine receptor; inhibition of in vitro ferti...
II. Characterization and development of the regional- and cellular-specific abnormalities in the epididymis of mice with beta-hexosaminidase A deficiency
II. Characterization regional- and cellular-specific abnormalities epididymis mice with beta-hexosaminidase A deficiency
2009/2/18
Beta-hexosaminidase (Hex) is a lysosomal enzyme that exists as two isoenzymes: Hex A (subunit structure alphabeta) and Hex B (betabeta). Its presence in the testis and epididymis suggests importan...
Construction and preliminary characterization of a series of mouse and rat testis cDNA libraries
Spermatogenesis Sertoli cells peritubular cells gene
2009/2/18
We have constructed a series of 23 cDNA libraries from mouse and rat testicular cells. These include libraries made from whole, intact adult testes; seminiferous tubule cells from adult testes; co...
Cloning and characterization of an androgen-dependent acidic epididymal glycoprotein/CRISP1-like protein from the monkey
Gutknecht J Brew K Spur N. Goodfellow PN. Cloning
2009/2/18
A cDNA encoding an acidic epididymal glycoprotein (AEG)-like, CRISP1 (cysteine-rich secretory protein) protein from the monkey (Macaca mullata) epididymis has been cloned and sequenced. The monkey A...